A CRISPR/DCAS9 TOOLKIT FOR FUNCTIONAL ANALYSIS OF MAIZE GENES

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Abstract Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants.The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present as a deactivated protein (dCas9).Results In this s

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Radioimmunological determination of serum 3 beta-hydroxy-5-cholenoic acid in normal subjects and patients with liver disease.

A radioimmunoassay for the determination of 3 beta-hydroxy-5-cholenoic acid in human serum has been developed, using 3 beta-hydroxy-5-cholenoyl-thyroglobulin as immunogen and 3 beta-hydroxy-5-cholenoylglycyl-125I histamine as radioactive Cigarette Lighter Power Adaptor ligand.The association constant was 6.3 X 10(8) l/mol.Cross reactivity with othe

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